| Congresso Brasileiro de Microbiologia 2023 | Resumo: 549-2 | ||||
Resumo:The intestinal microbiota may undergo changes due to the damage caused by chemotherapy treatment, facilitating the dissemination of opportunistic multiresistant microorganisms resistant to antimicrobials during the treatment of cancer patients. In this context, the objective of this study was to evaluate the susceptibility of bacterial isolates obtained from the feces of cancer patients treated at a Reference Center in the state of Rio Grande do Norte against the antibacterials ciprofloxacin and ceftriaxone. For this, all research participants answered a questionnaire about socioeconomic conditions, eating habits and hygiene, and use of antibiotics, and the stool samples obtained were plated on Nutrient Agar (AN), MacConkey Agar (MC), and Salmonella-Shigella (SS) agar and performed the MIC and MBC of the antimicrobials ciprofloxacin and ceftriaxone considering concentrations from 0.06μg/mL to 128μg/mL. The collected data were analyzed using the chi-square test, ANOVA, and Newman-Keusl test. Most patients declared themselves white (60%), female (93%), urban area residents (93%), had completed high school (30%) and most (50%) used the antibiotic amoxicillin before the beginning of the study. The most frequent age group observed was between 46 and 65 years (53.3%) and 83.3% of the patients had breast cancer. Regarding the stage of cancer, 30% had cancer in stage III of the disease and 33.3% were unable to inform, with a higher symptomatologic incidence of vomiting (88.7%), followed by diarrhea (66.7%), and nausea (50%). Considering the MIC, ciprofloxacin showed inhibitory activity in 90% of the isolates at a concentration of 16 μg/mL (MIC90) and 50% inhibition at a concentration of 8 μg/mL (MIC50). Regarding ceftriaxone, the bacterial samples showed MIC with inhibitory activity of 90% of the isolates at a concentration of 8 μg/mL (MIC90) and 50% inhibition at concentrations of 4 μg/mL (MIC50). In the control group, the MIC 1 μg/mL of ciprofloxacin and 4 μg/mL of ceftriaxone inhibited 100% of isolates, confirming the change in the MIC of fecal bacterial samples from cancer patients. It is concluded that the bacterial isolates obtained from cancer patients undergoing chemotherapy treatment showed a higher percentage of resistance to the tested antimicrobials when compared to the group of control individuals. Palavras-chave: intestinal microbiota, cancer, resistance, chemotherapy treatment, opportunistic bacteria Agência de fomento:Universidade Federal do Rio Grande do Norte | |||||